Figure 2.

Expression of C5aR1 in human embryonic stem cell-derived rosettes. A, Immunocytochemistry of human neural rosettes showing staining for neural marker TUBB3, tight junction marker ZO-1, and C5. C5aR1 localizes to the apical membrane, colocalizing with PKCζ, but not markers of cilia (Arl13b) or tight junctions (NCAD). Negative controls are shown at top right. Scale bar, 20 μm. B, Transcript expression as hES cells (day 0) are differentiated through the cortical rosette stage (day 28) to a mature neuronal lineage. C5AR1 expression is highest at the rosette stage of neuronal differentiation. C, hC5a is detected through ELISA within the lysate of rosette cultures and is not derived from the exogenous extracellular matrix (Matrigel). *p ≤ 0.05, **p ≤ 0.01.