Table 1.

PCR primer sequences and conditions

Name	Species	Use	Forward (5′–3′)	Reverse (5′–3′)	Annealing temperature (°C)	Product size (bp)
mC5aR1	Mouse	RT-PCR	ATGCTGATGCTGATGCTGATCG	ATGCTGATGGCTGATCGTCGGATGCTGAT	60	562
mActB	Mouse	RT-PCR	GTGGGCCGCCCTAGGCACCAG	CTCTTTGATGTCACGCACGATTTC	60	103
mC5aR1	Mouse	qPCR	GGGATGTTGCAGCCCTTATCA	CGCCAGATTCAGAAACCAGATG	60	131
mSox2	Mouse	qPCR	TAGAGCTAGACTCCGGGCGATGA	TTTCGTGGTCTTGTTTAAGGCAA	60	296
m18s	Mouse	qPCR	GATCCATTGGAGGGCAAGTCT	CCAAGATCCAACTACGAGCTT	60	103
hC5AR	Human	qPCR	TCCTTCAATTATACCACCCCTGA	GGAAGACGACTGCAAAGATGA	60	139
hC5	Human	qPCR	ACTGAATTTGGTTGCTACTCCTC	GTATTACTGGGACTCCTCCTACC	60	110
hACTB	Human	qPCR	GCGGGAAATCGTGCGTGACATT	GATGGAGTTGAAGGTAGTTTGGTG	60	232
hCDH2	Human	qPCR	ATCAACCCCATACACCAGCC	GTCGATTGGTTTGACCACGG	60	128