Table 1.
Mouse | Upstream (5′-3′) | Downstream (5′-3′) | Annealing temperature (°C) |
---|---|---|---|
Bmal1fl/fl mouse (floxed allele) | ACTGGAAGTAACTTTATCAAACTG | CTGACCAACTTGCTAACAATTA | 56 |
Cre mouse (cre recombinase) | GAACCTGATGGACATGTTCAGG | AGTGCGTTCGAACGCTAGAGCCTGT | 62 |
Nestin-GFP+ mice | ATCACATGGTCCTGCTGGAGTTC | GGAGCTGCACACAACCCATTGCC | 64 |
Bmal1-deficient mouse (WT allele) | CAAACCTGGTCGTCTGGAAT | GTCCTCCCCAAAAGGTGAAT | 64 |
Bmal1-deficient mouse (mutant allele) | CTCATCTGCTTATCTGCTCTGGGG | GGGGATTTCCATCTGTGTTTAC | 64 |