Figure 3.

HCM abolishes AβOs binding to hippocampal neurons and synapse loss. Mature mouse hippocampal neurons (19–21 DIV) were maintained for 30 min in the absence (A) or presence (B) of HCM, or CM from human astrocytes previously primed by AβO (HCM AβO; C), and were then exposed for 3 h to 500 nm AβOs. After this period, AβO binding (A–D) and density of synapses (E, F) were analyzed by quantification of the number of NU4 and synaptophysin/PSD-95 puncta, respectively. Fifteen images were acquired from duplicate coverslips for each experimental condition. Scale bar, 10 μm. *p < 0.050, ***p < 0.001, one-way ANOVA followed by Tukey's post hoc tests; n = 3 experiments with independent neuronal cultures; 90–100 cells were analyzed per experimental condition.