Figure 11.

IL-1β regulation of dendrite morphology is mediated by IL1RAPL1. A, Hippocampal neurons from WT and Il1rapl1-KO mice were infected at DIV 1 with lentivirus expressing GFP and treated with 10 ng/ml IL-1β or vehicle for 72 h before being fixed at DIV 5. GFP signal was used for the morphological analysis of the cells. B, The graphs show the quantification of average dendrite length and total number of primary dendrites. Ten to 15 neurons were analyzed for each genotype and treatment in three independent experiments. All values represent mean ± SEM. *p < 0.05 vs vehicle, $ < 0.05, $$ < 0.005 vs IL1RAPL1 WT vehicle, ANOVA followed by Dunnet's post hoc test. C, Hippocampal neurons from WT and Il1rapl1-KO mice were infected at DIV 8 with lentivirus expressing GFP and treated with 10 ng/ml IL-1β or vehicle for 72 h before being fixed at DIV 14. GFP signal was used for the morphological analysis of the cells. D, Graphs show the quantification of branching points. Two to 15 neurons were analyzed for each genotype and treatment in three independent experiments. All values represent mean ± SEM. **p < 0.005 vs vehicle, $$ < 0.005 vs IL1RAPL1 WT vehicle, ANOVA followed by Dunnet's post hoc test.