Figure 7.

IL1RAPL1 regulates dendrite arborization complexity before synaptogenesis. A, Hippocampal neurons were transfected at DIV 1 with GFP alone or in combination with HA-IL1RAPL1, HA-IL1RAPL1 C31R, HA-IL1RAPL1Δ8, or HA-IL1RAPL1 I643V, and were fixed and stained at DIV 4 with anti-HA antibody. GFP signal (left) was used for the morphological analysis of the cells. B–E, The graphs show the quantification of primary (B) and secondary (C) dendrites, and total (D) and average (E) length of primary and secondary dendrites of neurons overexpressing IL1RAPL1 constructs. Ten to 15 neurons were analyzed for each construct in three independent experiments. All values represent mean ± SEM. *p < 0.05, ***p < 0.005 vs GFP, ANOVA followed by Dunnet's post hoc test.