Figure 2.

Inducible deletion of Cox10 and specific reporter gene recombination. A, GLASTCreERT2:Coxflox/flox mice (age P30) were tamoxifen injected for 5 consecutive days and analyzed months later as indicated. B, Schema of the floxed Cox10 gene with the excision of exon 6 (not drawn to scale). Arrows indicate the location of PCR primers. C, Verification of Cox10 genomic recombination in mutant brains and cerebelli at the age of 3 months. D, Estimated recombination efficiency of GLASTCreERT2 mice in the cortex (ctx) and cerebellar subregions (pcl, PC layer; gcl, granule cell layer; wm, white matter) by analysis of R26R-EYFP reporter gene expression. Astrocytes, identified by S100β colabeling, were up to 93% recombinant (mean ± SEM). E, Virtually all BG marked with S100β (top: red channel) expressed EYFP (middle: green channel, immunostained). Black and white images have been pseudocolored in the bottom panel. Scale bars, 50 μm.