Figure 5.

Survival of BG cells in conditional Cox10 mutant mice. A, As shown by EdU labeling, mitotic cells (in red) and S100β-positive astrocytes (in green) were not costained in the cerebellum of Cox10 mutant and control mice (arrowhead indicates a mitotic nonastrocytic cell). Scale bar, 20 μm. B, When quantified, the total number of EdU-positive cells is not different in the mutant cerebellum. Data are shown as mean ± SEM. cer. sec., Cerebellar section. C, D, No signs of cell death in the cerebellum were observed by TUNEL staining (age 3 months; C), in contrast to a positive control (D), where Cox10 mutant neurons undergo apoptosis in the forebrain of CKIIα-Cre*Cox10fl/fl mice (age 4 months). Scale bar, 100 μm. n.s., Not significant.