Figure 7.

BDNF overexpression promoted adult DG neurogenesis. A, Decreases of BDNF levels in dDG or vDG of mice with chronic inflammatory pain were reversed by EE-VEx. Representative Western blots of BDNF were shown above the corresponding histogram. n = 7 in each group. *p < 0.05, **p < 0.01 vs BH plus saline; ^#p < 0.05, ^##p < 0.01 vs BH plus CFA, t test. B, Overexpressing BDNF with viral vectors. n = 5 in each group. *p < 0.05, **p < 0.01, t test. C, Representative images showing virus expression (indicated by EGFP) in the dDG (top) or the vDG (bottom). Scale bars, 500 μm. D, E, BDNF overexpression increased the number of BrdU-positive cells in mice with chronic inflammatory pain. F, G, BDNF overexpression increased the dendrite complexity of DCX-labeled immature granular cells in mice with chronic inflammatory pain. Scale bars, 100 μm. n = 7 in each group. *p < 0.05, t test.