Figure 4.

Asymmetric localization of GPM6a at stage 1 is before that of Par3. A, LN induced the asymmetric localization of GPM6a but not of Par3 in stage 1 neurons. Immunofluorescence analysis of the localization of endogenous GPM6a (red) and Par3 (green) in cortical neurons cultured on PLL or LN 30 min after plating (stage 1). GPM6a but not Par3 was asymmetrically localized at this stage. Scale bars, 10 μm. B, C, Cumulative angular probability distribution of the immunofluorescence of GPM6a (red) and Par3 (green) that is shown in A in stage 1 cortical neurons on PLL (blue) or LN (red).