Figure 4.

Protective effect of HDACIs on mitochondrial function in HD striatal cells. Oxygen consumption by wild-type and mutant cells was measured in an O₂ Clark electrode, after 24 h of incubation with PB (250 μm), SB (500 μm), and DCA (3 mm) before (A) and after (B) sequential addition of oligomycin (2.5 μg/ml) and FCCP (2.5 μm) and compared with untreated controls. Dotted lines in A represent average control basal values for wild-type and mutant cells and dotted lines in B represent control FCCP-stimulated respiration of wild-type and mutant cells. C–E, Energy metabolism in wild-type and mutant cells was evaluated by measurement of ATP (C), ADP (D), and AMP (E) levels by high-performance liquid chromatography after 24 h of incubation with PB and SB. Results are expressed as mean ± SEM of 4–5 independent experiments. Statistical analysis was performed by two-way ANOVA, followed by Bonferroni post hoc test. A significant effect of the treatment was found in A [F_{(3, 28)} = 9.102, p = 0.0002], B [F_{(3, 28)} = 4.291, p = 0.0134] and D [F_{(2, 15)} = 4.024, p = 0.0399], with *p < 0.05, **p < 0.01, ***p < 0.001 compared with the respective control. ^tp < 0.05, ^ttp < 0.01, compared with the respective control, by the Student's t test.