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. 2017 Sep 6;37(36):8830–8844. doi: 10.1523/JNEUROSCI.0730-17.2017

Figure 2.

Figure 2.

Loss of motor neurons and gliosis in GLAST+/−/GLT1-cKO mice. A, ChAT immunofluorescence of the lumbar ventral horn in control and GLAST+/−/GLT1-cKO mice at 5, 7, 9, and 20 weeks of age. Scale bar, 50 μm. B, Quantification of ChAT-positive motor neurons from 5 to 9, and 20 weeks of age of control mice (P5W, n = 4; P6W, n = 4; P7W, n = 3; P8W, n = 3; P9W, n = 4; P20W, n = 3) and GLAST+/−/GLT1-cKO mice (P5W, n = 5; P6W, n = 4; P7W, n = 4; P8W, n = 3; P9W, n = 5; P20W, n = 4). *p < 0.05, **p < 0.05, ***p < 0.001. n.s., not significant (unpaired two-tailed t test). C, NeuN immunofluorescence in the lumbar dorsal horn from control and GLAST+/−/GLT1-cKO mice at 20 weeks of age. Scale bar, 200 μm. D, Quantification of NeuN-positive neurons in the superficial dorsal horn (laminae I–IV) of the spinal cord at 5, 7, 9, and 20 weeks of control mice (P5W, n = 3; P7W, n = 4; P9W, n = 3; P20W, n = 3) and GLAST+/−/GLT1-cKO mice (P5W, n = 3; P7W, n = 4; P9W, n = 4; P20W, n = 3) mice. E, GFAP immunofluorescence in the lumbar ventral horn from control and GLAST+/−/GLT1-cKO mice at 5, 7, 9, and 20 weeks of age. Scale bar, 100 μm. F, Quantification of GFAP-positive cells in the lumbar ventral horn from 5 to 9, and 20 weeks of age of control mice (P5W, n = 3; P6W, n = 3; P7W, n = 4; P8W, n = 3; P9W, n = 3; P20W, n = 3) and GLAST+/−/GLT1-cKO mice (P5W, n = 3; P6W, n = 3; P7W, n = 4; P8W, n = 3; P9W, n = 3; P20W, n = 3) mice. *p < 0.05, **p < 0.01, ***p < 0.001. G, CD68 immunofluorescence in the lumbar ventral horns from control and GLAST+/−/GLT1-cKO mice at 5, 7, 9, and 20 weeks of age. Scale bar, 100 μm. H, Quantification of CD68-positive cells in the lumbar ventral horn from 5 to 9, and 20 weeks of age of control mice (P5W, n = 3; P6W, n = 3; P7W, n = 4; P8W, n = 3; P9W, n = 3; P20W, n = 3) and GLAST+/−/GLT1-cKO mice (P5W, n = 3; P6W, n = 3; P7W, n = 4; P8W, n = 3; P9W, n = 3; P20W, n = 3). *p < 0.05. All data are expressed as the mean ± SEM. n.s., Not significant (unpaired two-tailed t test).