Figure 4.

Loss of ErbB4 in striatum resulted in the upregulation of GABA_A receptor α1 subunit. A, B, GABA_AR α1 was upregulated in the cKO group with immunoblots and quantification of GABA_A receptor subunits α1, α2, and β2 in the striatum of Con and cKO mice (for GABA_ARα1, n = 5; for GABA_AR α2, n = 4 Con and 3 cKO; for GABA_ARβ2, n = 3). C, D, Immunoblots and quantification of inhibitory postsynaptic protein gephyrin and excitatory postsynaptic proteins GluN2A, GluN2B, and GluN1 in cKO striatum showing no change (for gephyrin, n = 4 Con and 3 cKO; for GluN2A, GluN2B, GluN1, n = 3), E–G, Immunoblots and statistical analysis of ErbB4 and GABA_ARα1 in positive areas tested in Erbb4 mutant mice including prefrontal cortex (PFC), hippocampus (Hp), hypothalamus (Hy), and striatum subregions the NAc and the CPu. GABA_AR α1 was increased in the NAc and the CPu, but not in the PFC, Hp, or Hy (for NAc, n = 5; for CPu, n = 3; for PFC, n = 3; for Hip, n = 3; for thalamus, n = 5). H, I, Analysis of GABA_ARα1 fluorescence intensity showing increased expression in cKO mice (n = 43 cells of 4 Con and 51 cells of 5 cKO). Statistical significance was calculated with two-tailed t test for unpaired data (B, D, F, G, I). All data are shown as mean ± SEM. NS, No significant difference.