Figure 9.

AAV-fDIO-Cre-EGFP virus could induce cre recombinase expression in a flp depended manner with high efficiency and reliability. A, B, AAV-fDIO-Cre-EGFP virus successfully induced tdTomato expression in Dlx5/6-flp;Ai65 mice after injected into the NAc core (AP + 1.34 mm, ML ± 0.8 mm and DV + 4.6 mm) and histograms of statistical results are shown (n = 428 cells of 3 AAV-fDIO-EGFP virus transfected mice and 844 cells of 4 AAV-fDIO-Cre-EGFP virus transfected mice). Arrowheads pointed to few tdTomato-negative neurons and GFP-positive neurons. Scale bar, 100 μm. C, D, DARPP32 in MSNs in virus transfected brain slices stained with DAPI indicating nuclei location and histograms of transfection efficiency and virus reliability (n = 1509 cells from 3 NAc-Con and 1854 cells from 3 NAc-cKO). Scale bar, 20 μm. E, Representative virus expression in the core of NAc (AP + 1.34 mm, ML ± 0.8 mm and DV + 4.6 mm) 3 weeks after injection. Scale bar, 1000 μm. In this experiment, all virus expression was restricted to the NAc core, so all data are included in the virus reliability analysis. Statistical significance was determined by two-tailed t test for unpaired data (D). All data are shown as mean ± SEM. NS, No significant difference.