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. 2017 Apr 5;37(14):3875–3886. doi: 10.1523/JNEUROSCI.3138-16.2017

Figure 5.

Figure 5.

Leptin-induced pSTAT3 signaling in the ARC and VMH of Lepr-null control, AgRP-LepR, rescue and Lepr-intact control animals. A, Representative Lepr-intact control section showed staining throughout both the vmARC and VMH. B, Representative AgRP-LepR rescue section showing staining in the vmARC. C, No pSTAT3 staining was observed in any region in Lepr-null animals. D, Quantification of leptin-induced pSTAT3 immunoreactivity, showing that leptin responsiveness was rescued in the vmARC of AgRP-LepR rescue mice (p = 0.0003 vs Lepr-null mice). In the VMH, the response to leptin remained undetectable in both AgRP-LepR rescue and Lepr-null animals (Lepr-intact controls, n = 7; AgRP-LepR rescue and Lepr-null, n = 8). ***p < 0.001. Scale bar, 200 μm.