Table 1.

DNA sequences of the primers used for RT-PCR and real-time PCR

Mouse cDNAs	RT-PCR methods	Primer sequences	GenBank accession no.
Il1b	Traditional	Forward, 5′-GCA ACT GTT CCT GAA CTC-3′	NM_008361
		Reverse, 5′-CTC GGA GCC TGT AGT GCA-3′
Il1b	Real-time	Forward, 5′-AGT TGC CTT CTT GGG ACT GA-3′	NM_008361
		Reverse, 5′-TCC ACG ATT TCC CAG AGA AC-3′
Nos2	Traditional	Forward, 5′-CCC TTC CGA AGT TTC TGG CAG CAG C-3′	NM_010927
		Reverse, 5′-GGC TGT CAG AGC CTC GTG GCT TTG G-3′
Nos2	Real-time	Forward, 5′-GCC ACC AAC AAT GGC AAC A-3′	NM_010927
		Reverse, 5′-CGT ACC GGA TGA GCT GTG AAT T-3′
Tnf	Traditional	Forward, 5′-CAT CTT CTC AAA ATT CGA GTG ACA A-3′	NM_013693
		Reverse, 5′-ACT TGG GCA GAT TGA CCT CAG-3′
Tnf	Real-time	Forward, 5′-ATG GCC TCC CTC TCA GTT C-3′	NM_013693
		Reverse, 5′-TTG GTG GTT TGC TAC GAC GTG-3′
Ccl4	Traditional and real-time	Forward, 5′-GCC CTC TCT CTC CTC TTG CT-3′	NM_013652
		Reverse, 5′-GTC TGC CTC TTT TGG TCA GG-3′
Orm1	Traditional	Forward, 5′-GCT GCA CAC GGT TCT TAT CA-3′	NM_008768
		Reverse, 5′-CCT CAG CAC TAT AAG GTG GGC-3′
Orm2	Traditional and real-time	Forward, 5′-TTG TCA TGG TGA GCC TCC TG-3′	NM_011016
		Reverse, 5′-ATG AAG GCC CCA TGC ATC TT-3′
Orm3	Traditional	Forward, 5′-AGA AGT AGA AAC CCT TCT CCA CC-3′	NM_013623
		Reverse, 5′-AAA GTG CAC AGG ATG GGG TA-3′
Il6	Traditional	Forward, 5′-CGG CCT TCC CTA CTT CAC AA-3′	NM_031168
		Reverse, 5′-TAA CGC ACT AGG TTT GCC GA-3′
Il6	Real-time	Forward, 5′-AGT TGC CTT CTT GGG ACT GA-3′	NM_031168
		Reverse, 5′-TCC ACG ATT TCC CAG AGA AC-3′
Gapdh	Traditional	Forward, 5′-ACC ACA GTC CAT GCC ATC AC-3′	NM_008084
		Reverse, 5′-TCC ACC ACC CTG TTG CTG TA-3′
Gapdh	Real-time	Forward, 5′-TGG GCT ACA CTG AGC ACC AG-3′	NM_008084
		Reverse, 5′-GGG TGT CGC TGT TGA AGT CA-3′