Figure 3.

Effects of PVT silencing on danger in the absence and presence of reward. A, Design and procedure for the two stages. B, Freezing behavior on the conditioning day for tone–shock pairings and for tone presentations on test in Stage I. C, Freezing behavior on the conditioning day for each of the clicker–shock pairings in Stage II. D, Defense and reward behavior during clicker presentations on the test day. Defense is percentage of observations where freezing was expressed. Reward is sum of approach behavior (lever press and food magazine entry). Inset shows reward approach across blocks of two test trials. E, Comparison of freezing behavior during tone presentations on test in Stage I and Stage II. Data are means ± SEM. *p < 0.05. Group sizes were as follows: eYFP, n = 8; hM4Di, n = 8.