Anti-LINGO1 antibody-induced OPC differentiation is inhibited by GSN siRNA. A, Western blot analysis of MBP and GSN protein levels in rat primary OPCs transfected with Ctrl or cGSN siRNA and then differentiated for 3 d in the presence of the anti-LINGO-1 antibody or a Ctrl Ab. B, Quantification of the Western blot of GSN in A with Image Studio software (LI-COR) and represented as the band intensity relative to actin. p = 0.0192 (PBS), p = 0.0011 (anti-LINGO-1), p = 0.0004 (Ctrl Ab) by two-tailed unpaired Student's t test. C, Quantification of the Western blot of MBP in A as done in B. p = 0.525 (PBS); p = 0.0052 (anti-LINGO-1); p = 0.1411 (Ctrl Ab). D, OPCs transfected with Ctrl or GSN siRNA were immunostained for MBP (green). Blue, DAPI. Scale bar, 50 μm. E, Quantification of myelin-like membrane area per cell shown in D. Average 4 fields per slide. n = 2 biological replicates. p = 0.7023 (PBS); p = 0.0247 (anti-LINGO-1); p = 0.1349 (Ctrl Ab); two-tailed unpaired Student's t test. F, Western blot analysis of MBP and GSN protein levels in primary OPCs transfected with Ctrl or pGSN-specific siRNA and then differentiated for 3 d. G, Quantification of pGSN mRNA expression level 48 h after siRNA transfection as in F. The expression in control siRNA-treated samples was set as 1. n = 2 biological replicates. p = 0.0127 by two-tailed unpaired Student's t test.