cGSN is downregulated in Shiverer mice spinal cord and brain and human MS brain tissues. A, Western blot analysis of MBP, MAG, MOG, GSN, CNPase, GAPDH, and actin levels in spinal cord and brain lysates of P30 Shiverer mice or wild-type littermates. B, Quantification of the Western blot of GSN in A, represented as band intensity relative to GAPDH. p = 0.0491 (spinal cord); p = 0.0487 (brain); two-tailed unpaired Student's t test. C, Representative Western blot analysis of LINGO-1 from brain lysates of 10 human MS patients and 10 control samples (patient IDs correspond to the numbers listed in Table 1). D, Representative Western blot analysis of GSN from brain lysates of 10 human MS patients and 10 control samples (patient IDs correspond to the numbers listed in Table 1). E, Quantification of the Western blots for LINGO-1 from brain lysates of 20 human MS patients and 20 control samples using Image Studio software (LI-COR). Data are presented as band intensities relative to loading control (GAPDH). p < 0.0001; two-tailed unpaired Student's t test. F, Quantification of the Western blots for GSN from brain lysates of 20 human MS patients and 20 control samples as done in E. p < 0.0001.