Figure 1.

KCTD hetero-oligomers are abundant in the adult mouse brain. A, Anti-KCTD12 and anti-KCTD16 antibodies (Schwenk et al., 2010) copurify KCTD12 and KCTD16 proteins in immunoprecipitation (iP) experiments with brain lysates of WT mice, as shown on immunoblots (IB). Control IPs with brain lysates of Kctd12^−/− and Kctd16^−/− mice (Metz et al., 2011) show that the antibodies are specific for the KCTD12 and 16 proteins. Asterisks indicate cross-reactions of the anti-KCTD16 antibody in the Input samples. B, Affinity depletion of KCTD12 from hippocampi of adult mouse brains using anti-KCTD12 antibodies leads to a codepletion of KCTD16 engaged in KCTD12/KCTD16 hetero-oligomers (depleted). Control IBs of β-tubulin III show that the neuron-specific marker protein is not depleted by anti-KCTD12 antibodies.