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. 2017 Feb 1;37(5):1162–1175. doi: 10.1523/JNEUROSCI.2181-16.2016

Figure 2.

Figure 2.

KCTD8, KCTD12, and KCTD16 form homo- and hetero-oligomers in transfected HEK293. A, KCTD domain structure. KCTDs contain T1, H1, and H2 homology domains that are not closely sequence-related with the T1 and C-terminal (C) domains of KCTD10. The H2 domains are selectively present in KCTD8 and KCTD16. B, KCTDs form homo-oligomeric complexes. Input lanes indicate the expression of FLAG- or Myc-tagged KCTD proteins in the cell lysates used for IP with anti-Myc antibodies. Proteins in the IPs were revealed by immunoblotting (IB) with anti-FLAG and anti-Myc antibodies. C, KCTD8, KCTD12, and KCTD16 form hetero-oligomers with each other, but not with KCTD10. Input lanes indicate expression of the FLAG- or Myc-tagged KCTD proteins in the cell lysates used for IPs with anti-Myc antibodies. Proteins in the IPs were revealed by IB with anti-FLAG and anti-Myc antibodies.