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. Author manuscript; available in PMC: 2019 Nov 22.
Published in final edited form as: Nature. 2019 May 22;569(7758):718–722. doi: 10.1038/s41586-019-1228-x

Extended data Table 1.

Binding affinities of human STING mutants with TBK1

Human STING (hSTING) Human TBK1 1–657, S172A
Kd (μM)
M0US6 TBK1 1–657, S172A
Kd (μM)
M0US6 TBK1 1–657
Kd (μM)
hSTING 155–379 (no cGAMP) ~230 ~260
hSTING 55–379 ~290 ~170
p-hSTING 155–379 11.0 9.1 13.3
p-hSTING 155.379 (no cGAMP) 20.0 12.5
p-hSTING 155–341 no binding no binding
p-hSTING 155–370 no binding no binding
p-hSTING 342–379 16.8 15.3
p-hSTING 342–370 no binding no binding
p-hSTING 353–379 22.6 25.1
p-hSTING 366–379 35.6 30.8
hSTING 366–379, F376W ~190
hSTING 155–379, T376E ~62.0
hSTING 356–379, T376E F378W ~50.0
hSTING 366–379, T376E F378W S379E ~127
hSTING 366–379, T376E F378W 3379M 43.9
hSTING 366–379, T376E F378W S379I ~59.0
hSTING 366–379, T376E F378W S379F 27.1
hSTING 356–379, T376E F376M S379W 2.6 2.7
hSTING 155–379, T376E F378M S379VV 1.4 1.2