Fig. 2.

Specific characterization of APA sites in X. tropicalis. a PCA analysis of 26 APA profiles related to five stages of embryos and two stages of adult male and female frogs. b Stage-based Spearman’s correlation estimates. c Classification of embryonic APA sites into maternal (OriA), maternal–zygotic (OriB), zygotic (OriC) and mixed (OriD) RNA sources of origin. Effects of APA origin on d site types and e genome neighborhoods. Effects of gender on f number of APA sites per gene and g site types and ratios between ARSs and NARSs. Effects of longitudinal stages on h APA basics, i site types and j genome neighborhoods