Skip to main content
. Author manuscript; available in PMC: 2019 Jun 27.
Published in final edited form as: Nat Protoc. 2018 Sep;13(9):2062–2085. doi: 10.1038/s41596-018-0032-7
Composition Volume (~500 ml) Final concentration Comments
Neurobasal A 480 ml
B27 supplement 10 ml 2% (vol/vol)
GlutaMax 5 ml 1% (vol/vol)
Penicillin/streptomycin 5 ml 1% (vol/vol)
EGF (20ug/mL stock) 500 μl 20 ng/ml For hSS and hCS. Only from day 6–25. Add just before use.
FGF2 (20ug/mL stock) 500 μl 20 ng/ml For hSS and hCS. Only from day 6–25. Add just before use.
BDNF (20ug/mL stock) 500 μl 20 ng/ml For hSS and hCS. Only from day 25–43. Add just before use.
NT3 (20ug/mL stock) 500 μl 20 ng/ml For hSS and hCS. Only from day 25–43. Add just before use.
IWP-2 (5mM stock) 500 μl 5 μM Only for hSS conditions from day 6 to day 24. Add just before use.
SAG (100μM stock) 500 μl 100 nM Only for hSS conditions from day 12 to day 24. Add just before use.
Allo-P (100μM stock) 500 μl 100 nM Only for hSS conditions from day 15 to day 24. Add just before use.
RA (100μM stock) 500 μl 100 nM Only for hSS conditions from day 12 to day 15. Add just before use.