| Composition | Volume (200 ml) | Final concentration |
|---|---|---|
| ddH20 | 170.4 ml | |
| 10x EBSS | 20 ml | 1x |
| 30% D(+)−Glucose | 2.4 ml | 0.46% (vol/vol) |
| 1M NaHCO3 | 5.2 ml | 26 mM |
| 50mM EDTA | 2 mL | 0.5 mM |
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