TABLE 1.

Markers that can be used to characterize hPSC-derived 3D spheroids.

Marker	Temporal expression	Spatial expression and specificity	Antibody (optimal Dilution)
SOX2	Throughout differentiation	Present in pluripotent stem cells, neural progenitors and astrocytes in both hCS and hSS	Cell Signaling Technologies, SOX2 (D6D9), cat. no. 3579S (1: 500)
PAX6	From ~day 20	Early neuroectoderm progenitors and dorsal pallium progenitors	DSHB, cat. no. PAX6 (1:250)
TBR2	From ~day 50	Intermediate progenitors	Abcam, cat. no. ab757520 (1/300)
HOPX	From ~ day 50	Outer radial glia	Santa Cruz, cat. no. sc-398703 (1:500)
TBR1	From ~ day 50	Deep layer neurons in hCS	Abcam, cat. no. ab31940 (1: 500)
CTIP2	From ~ day 50	Deep layer neurons in hCS, but also expressed in hSS neural cells	[25B6] Abcam, cat. no. ab18465 (1: 300)
SATB2	From ~ day 100	Superficial layer neurons in hCS	[SATBA4B10] Abcam, cat. no. ab51502 (1: 400)
NKX2.1	From ~ day 25	Ventral forebrain progenitors (hSS)	Santa Cruz, cat. no. sc-13040 (1: 200)
GAD67	From ~ day 50	GABAergic neurons in hSS; sporadic expression in hCS	Millipore, cat. no. MAB5406 (1: 1000)
GABA	From ~ day 50	GABAergic neurons in hSS; some hCS cells can also express it transiently	Sigma-Aldrich, cat. no. A2052 (1: 1000)
SST	From ~ day 50	Interneuron subtype in hSS	Millipore, cat. no. MAB354 (1: 200)
PV	From ~ day 200	Interneuron subtype in hSS	Swant, cat. no. PV27 (1: 6000), Millipore, cat. no. MAB1572 (1: 1000)
CR	From ~ day 50	Interneuron subtype in hSS; sporadic expression in hCS	Swant, cat. no. CR7697 (1: 1000)
CB	From ~ day 50	Interneuron subtype in hSS; sporadic expression in hCS	Swant, cat. no. CB38 (1: 1000)
NEUN	From ~ day 75	Neurons in both hCS and hSS	Millipore, cat. no MAB377 (1: 500)
MAP2	From ~ day 30	Neurons in both hCS and hSS	Synaptic Systems, cat. no. 188004 (1:10.000)
GFAP	From ~ day 50	Astrocytes in both hCS and hSS, also marks radial glia	DAKO, cat. no. Z0334 (1: 1000)