Fig 5. Strategy for the identification of protein responsible for the glutenase activity in C. taeanense strain 2RA3.

A, Gliadin zymography of culture supernatant. Lane 1, molecular weight marker, lane 2, strain 2RA3. B, Nano-ESI-Q-TOF mass-spectrometer analysis of the potential active band after trypsin treatment; C, Amino acid sequence of annotated protease in the genome of C. taeanense strain 2RA3 responsible for the activity detected in the excised band.