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. 2019 Jun 27;14(6):e0218346. doi: 10.1371/journal.pone.0218346

Fig 7. Characterization of the recombinant PEP.

Fig 7

A, Hydrolysis of Suc-Ala-Pro-pNA and Z-Gly-Pro-pNA by recombinant PEP. B, Activity profiles measured using Suc-Ala-Pro-pNA at various concentrations. C, The effect of pH was determined by incubating the enzyme in citrate/disodium phosphate buffer (pH 2–8), Tris-HCl (9–10), and glycine/NaOH (pH 11–12) for 30 min with 1.5 mM Suc-Ala-Pro-pNA. D, Hydrolysis of 1.5 mM Suc-Ala-Pro-pNA at various temperatures. The activity for optimum temperature was determined under conditions at 20–70°C and optimum pH for 30 min.