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. 2019 Jun 17;15(6):e1007893. doi: 10.1371/journal.ppat.1007893

Fig 4. Oleate is required for host resistance to P. aeruginosa infection.

Fig 4

A and C. P. aeruginosa pathogenesis assays of wild-type and fat-6(tm331);fat-7(wa36) (A) or fat-2(wa17) (C) animals grown on control media or oleate-supplemented media, as indicated. Animals were transferred at the L4 stage to plates containing P. aeruginosa. Assay plates were not supplemented with oleate. fat-6(tm331);fat-7(wa36) animals are more susceptible to killing by P. aeruginosa (p<0.01). Oleate supplementation rescues the enhanced susceptibility to pathogen phenotype of the fat-6(tm331);fat-7(wa36) mutant (p<0.01). fat-2(wa17) animals are not hypersusceptible to killing (p = not significant). Data are representative of at least two trials. Sample sizes, mean lifespan, and p values for all trials are shown in S2A Table. Significance was determined using Kaplan-Meier survival curves and log-rank tests. B and D. Expression of the indicated genes measured by qRT-PCR in wild-type, fat-6(tm331);fat-7(wa36) double-mutant, or fat-2(wa17) animals exposed to P. aeruginosa at the L4 stage for 6 hours. Data are the average of three independent replicates, each normalized to a control gene and presented as fold induction in animals of the indicated genetic background infected with P. aeruginosa vs. animals fed the standard bacterial food source (E. coli OP50). Error bars show standard deviation. Statistical significance was determined using t-tests. * p<0.05. E and F. P. aeruginosa pathogenesis assays of wild-type and fat-6(tm331);fat-7(wa36) exposed to control RNAi bacteria, tir-1(RNAi) or zip-2(RNAi) bacteria, as indicated. fat-6(tm331);fat-7(wa36)+ tir-1(RNAi) animals are significantly more susceptible than fat-6(tm331);fat-7(wa36) mutants and tir-1(RNAi) animals (p<0.05). In addition, fat-6(tm331);fat-7(wa36)+ zip-2(RNAi) animals are significantly more susceptible than fat-6(tm331);fat-7(wa36) mutants and zip-2(RNAi) animals (p<0.05). Data are representative of at least two trials. Sample sizes, mean lifespan, and p values for all trials are shown in S2A Table. Significance was determined using Kaplan-Meier survival curves and log-rank tests.