Table 2. Interrater reliability as defined by kappa agreement values (Cohen’s kappa, κ) including standard error of κ, 95% confidence interval (CI) of κ and significance of κ; and coefficient of determination (R2) for qPCR vs MT-PCR to determine closeness of data to fitted linear regression line.
Standard errors (SE) range from 0.0361 to 0.0465 with a 95% confidence interval ranging from 0.808 to 1.00 among all tests. All p-values for κ are <0.00001 assuming interrater reliability measurements to be significant. R2 values are given in percentages of the total number of samples validated for each STH species (N). Only samples deemed infection positive by both methods were included in this analysis (1 Ascaris lumbricoides and 3 Necator americanus outliers were removed (highlighted in Fig 2)). All Ancylostoma ceylanicum positive infections represented co-infections with Necator americanus and were subsequently discarded from this analysis.
| Total Agreement (% Agreement) |
Sensitivity (%) | Specificity (%) | κ | SE of κ | 95% CI of κ | p-value of κ | N | qPCR vs MT-PCR R2 (%) |
|
|---|---|---|---|---|---|---|---|---|---|
| A. lumbricoides | 431 (93.29) | 83.33 | 100.00 | 0.8566 | 0.0460 | 0.808–0.905 | p<0.00001 | 155 | 97.36 |
| T. trichiura | 461 (99.78) | 100.00 | 99.78 | 0.9513 | 0.0465 | 0.856–1.000 | p<0.00001 | 10 | 75.48 |
| N. americanus | 739 (96.73) | 91.42 | 99.60 | 0.9268 | 0.0361 | 0.899–0.955 | p<0.00001 | 245 | 80.63 |
| Ancylostoma spp. | 763 (99.87) | 96.88 | 100.00 | 0.9834 | 0.0362 | 0.951–1.000 | p<0.00001 | - | - |