Skip to main content
. 2019 Jun 17;13(6):e0007431. doi: 10.1371/journal.pntd.0007431

Fig 2. The assessment of purification steps by size-exclusion chromatography.

Fig 2

Analysis was performed on TSK-Gel G3000SWXL column (7.8 × 300 mm) with 0.1 M phosphate-sulphate running buffer, pH 6.6, at a flow rate of 0.5 mL min-1. Heat-treated plasma (A). IgG fraction—supernatant from 2% caprylic acid precipitation before (crude IgG; B) and after diafiltration using a 100 kDa membrane (pure IgG; C). F(ab')2 fraction produced by pepsin digestion of IgG preparation before (crude F(ab')2; D) and after diafiltration using a 50 kDa membrane (pure F(ab')2; E). Ultrapure F(ab')2 preparation—flow-through fraction from anion-exchange chromatography performed at pH 5.0 (F). Detection: UV at 280 nm.