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. 2019 Jun 17;13(6):e0007431. doi: 10.1371/journal.pntd.0007431

Fig 6. The assessment of IgG/F(ab')2 aggregation by size-exclusion chromatography.

Fig 6

Analysis was performed on TSK-Gel G3000SWXL column (7.8 × 300 mm) with 0.1 M phosphate-sulphate running buffer, pH 6.6, at a flow rate of 1 mL min-1. IgG substrate—supernatant from 2% caprylic acid precipitation before diafiltration (A). IgG fraction acidified to pH 3.2 in the presence of 2% caprylic acid (V/V) and incubated at +4 °C (B) or 37 °C (C). F(ab')2 fraction produced by pepsin digestion in the presence of caprylic acid (D). Detection: UV at 280 nm.