Table 2. Characterisation of the unbound fractions following incubation of F(ab)2 preparation containing pepsin with UNOsphere Q stationary phase under variuos pH conditions.
pH | Pepsin in unbound fraction | F(ab')2 yield [%] |
---|---|---|
4.0 | + | 97.6 |
5.0 | - | 89.2–93.0 |
6.0 | - | 86.2–91.3 |
Pepsin traces were detected by SDS-PAGE ("+" denotes presence and "-" denotes absence). Active principle yield [%] was calculated according to ELISA results.