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. 2019 Jun 17;129(7):2807–2823. doi: 10.1172/JCI127277

Figure 5. Tissue-independent effect of Drp1S600A on mitochondrial fission in diabetic mice.

Figure 5

Hippocampal neurons and TA muscle cells from mice of different groups were fixed for histology and TEM analysis. (A) Representative TEM images. Scale bar: 500 nm. (B) Quantification of mitochondrial morphology from TEM images. (C) Immunofluorescence staining of paraffin-embedded hippocampal tissue. Left panels show mitochondria stained for Tomm20 (red). Middle panels show gray-scale images of p-Drp1S600 staining. All gray-scale images were captured with identical confocal settings, and images were manipulated simultaneously and equally for each tissue. Right panels show total Drp1 staining (green). Scale bar: 25 μm. (D) Immunofluorescence staining of TA muscle cells as performed in C. Scale bar: 25 μm. (E) Quantification of p-Drp1S600 staining intensity from sections of hippocampus and TA muscle immunostained with anti–p-Drp1S600. Representative images are from a sampling of 3 animals. ***P < 0.001 and ****P < 0.0001, by 1-way ANOVA with Tukey’s multiple comparisons test. Results are presented as the mean ± standard error of the mean (n = 3–5/group).