Figure 2. Decreased IAV-specific CD4⁺ T cells in the lungs of Nlrc4^–/– mice 7 days after infection.

(A–F) Mice were infected with a 0.5 LD₅₀ inoculum of IAV, and lung CD4⁺ T cell subsets were enumerated by flow cytometry on the indicated days (A, B, E, and F) or on day 7 after infection (C and D). Data are from 3 (C and D, n = 12–16 per group) or 4 (A, B, E, and F, n = 13–20 per group) independent experiments. Error bars represent the SEM. *P < 0.05, ***P < 0.001, and ****P < 0.0001, by 2-tailed Student’s t test.