Figure 1. Trx1 regulates the T cell response to alloantigen in vivo.

Naive CD4⁺ and CD8⁺ T cells (CD25^–CD44^–CD62L⁺) were purified separately from WT and Trx1-Tg mice on B6 background and pooled with 2:1 (CD4/CD8) ratio. Combined T cells were labeled with CFSE and injected i.v. into lethally irradiated BALB/c mice at 2 × 10⁶ per mouse. Four days after cell transfer, spleens were collected from recipient mice and subjected to cell counting and FACS staining. (A) CD4⁺ and CD8⁺ cells are shown among gated live donor cells (H2K^b+). (B and D) CFSE dilution and percentage IFN-γ⁺ cells are on gated donor CD4⁺ or CD8⁺ cells. (C and E) Data shown are from 2 combined experiments of 6–7 mice per group. The mean ± SD is depicted for 6–7 mice per group. Significance was determined by Student’s t test. **P < 0.01 and ***P < 0.001.