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. 2019 May 28;129(7):2730–2744. doi: 10.1172/JCI126912

Figure 2. Gal1R-dependent pharmacodynamic differences of MOR agonists.

Figure 2

(A–E) Representative concentration-response experiments of ligand-induced BRET changes in HEK-293T cells transfected with the MOR fused to Rluc and the α subunit of the Gi1 protein fused to YFP. Values represent the mean ± SEM of triplicates. The effect of increasing concentrations of the MOR agonists morphine (A), EM1 (B), DAMGO (C), fentanyl (D), and methadone (E) were evaluated without cotransfection with Gal1R, in the absence or presence of the Gal1R/Gal2R antagonist M40 (red and green curves, respectively), or with cotransfection with Gal1R, with or without M40 (blue and purple curves, respectively). (F and G) Comparison of the Emax and E50 values obtained with and without cotransfection with the Gal1R. **P < 0.01 versus transfection with MOR alone; unpaired, 2-tailed t test (F); **P < 0.01 versus transfection with MOR alone; 2-tailed Mann-Whitney U test (G). Emax and EC50 values are shown as dots, presented with the mean ± SEM (F) or median with interquartile ranges (G) (n = 5–10 triplicates/group).