Skip to main content
. 2019 Jun 10;129(7):2920–2931. doi: 10.1172/JCI124829

Figure 5. Ablation of bone marrow DCs activates sinusoidal endothelial cells.

Figure 5

(A) Sinusoidal endothelial cells were sorted from Zbtb46dtr bone marrow chimeras treated with PBS or DT (1 day) and RNA-sequencing was performed. t-SNE analysis of PBS- (n = 4 mice) and DT-treated mice (n = 3 mice). (B, C) Gene set enrichment analysis (GSEA). (D) Fragments per kilobase (FPKM) values for all expressed chemokine receptors in sinusoidal endothelial cells. *False discovery rate < 0.05. Significance was determined using the Partek gene-specific analysis (GSA) algorithm. (E, F) Expression of CXCL1 (E) and CXCL2 (F) mRNA relative to β-actin mRNA in the bone marrow of Zbtb46dtr chimeras following treatment with PBS (n = 6 mice) or DT for 1 day (1D-DT, n = 5 mice) or 6 days (6D-DT, n = 5 mice). Data are mean ± SEM. ***P < 0.001 compared with PBS-treated mice. Significance was determined using ANOVA with Tukey’s Honest Significant Difference post hoc analysis for E and F.