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. 2019 Jun 13;8:e46300. doi: 10.7554/eLife.46300

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© 2019, Richter et al

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Figure 6. — Recombinant GST- and 6xHIS-tagged GBP (A) and GluTR1 (B), respectively, were incubated with hemin-agarose (HA) or unmodified agarose (A), washed, eluted and subjected to electrophoresis on a 12% SDS-polyacrylamide gel. One microgram of the input was loaded as a reference (I). Note that only GBP binds to the HA. Arrows on the right mark the position of the GST and 6xHIS-tagged proteins.