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. 2019 Jun 13;8:e46300. doi: 10.7554/eLife.46300

Figure 8. Proposed model for heme-dependent GluTR1 degradation by Clp protease.

In the absence of heme, GBP binds to the ‘regulatory domain’ (RED, formerly ‘heme binding domain’) and prevents degradation of GluTR1. When heme levels increase, release of GBP from GluTR1 enables the binding of Clp components to the latter and its concomitant proteolytic degradation.

Figure 8.

Figure 8—figure supplement 1. Alignment of the amino-acid sequences of Arabidopsis GluTR1 (encoded by HEMA1), GluTR2 (HEMA2) and the regulatory domain of GluTR1 (RED, formerly heme-binding domain - HBD).

Figure 8—figure supplement 1.

Figure 8—figure supplement 2. Distribution of GBP and regulatory domain (RED, formerly heme-binding domain - HBD)-containing GluTR isoforms in photosynthetic organisms.

Figure 8—figure supplement 2.

Protein BLAST analyses (https://blast.ncbi.nlm.nih.gov/Blast.cgi) were performed using either the full-length AtGBP (AT3G21200, left) or the RED of AtGluTR1 (right). The top 100 hits were used to build the trees. Note that both GBP and the RED-containing GluTR isoform were only found in land plant species. The query entries used are highlighted in blue.