Fig. 3.

Ectopic overexpression of FDPS in PTEN-deficient PCa cells increases proliferation and growth advantage. a Schematic representation of predicted FDPS-mediated mechanism(s) in PCa cells. b and c p3Xflag-CMV-10-FDPS construct and empty vector were stably transduced with similar units in PTEN-sufficient 22Rv1 and PTEN-deficient C4-2B PCa cells. Subsequently, stably transfected cells were cultured with selection medium containing G418 at 300 μg/ml and clones were selected and analyzed for FDPS positivity using b, Western blot and c, confocal analysis. d Box plot shows an increase in the average number of colonies in C4-2B PCa cells stably transfected with FDPS cDNA as compared to 22Rv1 cells. e Cells with FDPS overexpression or vector transduction were assessed for proliferative kinetics by a growth kinetics assay. Cells were measured for 7 days using a cellometer auto 1000 cell counter. The average number of FDPS-overexpressed cells was significantly higher compared to vector-transduced PCa cells.