Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2020 Jun 25.

Published in final edited form as: Biochemistry. 2019 Jun 7;58(25):2834–2843. doi: 10.1021/acs.biochem.9b00393

Figure 2. — Time-dependent inactivation of NDM variants. A) Dilution assays indicate time-dependent irreversible inactivation of NDM-1 by 1 (, 10 μM), in contrast to a control incubation that omits the inactivator (). B) Inactivation of NDM-1 by 1 (, 10 μM) is followed by addition of a second aliquot of fresh enzyme to the same preincubation tube (), with the activity immediately after addition reset to 100%. A control incubation omits the inactivator (). C) Dilution assays indicate time-dependent irreversible inactivation of C208D NDM-1 by 1 (, 10 μM). The control incubation with inactivator omitted (), indicates lower stability of the C208D NDM-1 variant that wild type NDM-1. D) Dilution assays indicate no time-dependent irreversible inactivation of K211A NDM-1 by 1 (, 10 μM), in comparison to a control incubation that omits the inactivator (). Fitting errors from incubations that showed appreciable inactivation were <15 %.

Inline graphic — Time-dependent inactivation of NDM variants. A) Dilution assays indicate time-dependent irreversible inactivation of NDM-1 by 1 (, 10 μM), in contrast to a control incubation that omits the inactivator (). B) Inactivation of NDM-1 by 1 (, 10 μM) is followed by addition of a second aliquot of fresh enzyme to the same preincubation tube (), with the activity immediately after addition reset to 100%. A control incubation omits the inactivator (). C) Dilution assays indicate time-dependent irreversible inactivation of C208D NDM-1 by 1 (, 10 μM). The control incubation with inactivator omitted (), indicates lower stability of the C208D NDM-1 variant that wild type NDM-1. D) Dilution assays indicate no time-dependent irreversible inactivation of K211A NDM-1 by 1 (, 10 μM), in comparison to a control incubation that omits the inactivator (). Fitting errors from incubations that showed appreciable inactivation were <15 %.