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. Author manuscript; available in PMC: 2019 Jun 27.
Published in final edited form as: Allergy. 2015 Aug 25;70(12):1651–1655. doi: 10.1111/all.12701

Figure 1.

Figure 1

Characterization of Exos-rFel d1. Expression of the surface markers HLA-DR, CD63, and CD81 on Exos and Exos-rFel d 1 was measured by flow cytometry and is shown as (A) histograms and % positive cells from one representative experiment of three independent experiments using exosomes from different healthy blood donors (isotype control in black), or as (B) the ratio of the mean fluorescence intensity (MFI). Results represent the mean ± SEM from three independent experiments. (C) The morphology of Exos-rFel d 1 was visualized by performing iEM on pools of Exos-rFel d 1 from five healthy blood donors using a mAb against CD81 (detected with a 10 nm gold particle-conjugated anti-rabbit Ab; indicated by arrows) and corresponding isotype control. (D) The mean particle size distribution from five different exosome batches for Exos, Exos/LPS, Exos-rFel d 1 and Exos + rFel d 1 analyzed by nanoparticle tracking analysis (NanoSight).