IgE reactivity to natural fish allergens. Nitrocellulose-blotted
natural allergen extracts from carp (A), cod (B),
and tuna (C) were incubated with sera from 12 children with
fish allergy (1-12; Co, positive control
serum from a patient with fish allergy), serum from a nonallergic subject
(N), or buffer alone (B). Bound IgE
was detected with iodine125–labeled anti-IgE antibodies
and visualized by means of autoradiography. Molecular weights (in
kilodaltons) are displayed on the left margin.