Dot-blotted wtCyp c 1 and mCyp c 1 were tested regarding IgE
reactivity with sera from patients with fish allergy (Co
and 13-27), serum from a nonallergic subject
(N), or buffer alone (B). IgE was
detected with an iodine125–labeled anti-IgE antibody, and
counts per minute values corresponding to wtCyp c 1– and mCyp c
1–bound IgE, as well as the percentage reduction of IgE binding to
mCyp c 1, are shown.