FIG 4.

GspD overexpression increases psp transcript levels and leads to PspA membrane association. Cultures were grown to mid-log phase when protein production was induced by the addition of arabinose for 1 h. (A) RNA was harvested and reverse transcribed. Transcript levels were normalized to the housekeeping gene recA. (B) Induced and uninduced cultures were fractionated into soluble and membrane fractions. Crp-FLAG and ToxR were used as cytoplasmic and membrane controls, respectively. Figure is representative of 3 experiments.