FIG 7.
The psp null strain shows reduced colonization in the zebrafish model of cholera infection but no defect in infant mice. (A) Infant mice were orally inoculated with a 1:1 ratio of 106 O395ΔlacZ and O395ΔpspFABC. (B) Zebrafish were incubated in water containing either 108 O395ΔlacZ or O395ΔpspFABC. After overnight infections, intestines were harvested and bacteria were plated for enumeration. Each data point represents data from one fish or mouse. (C) The water was tested for mucin concentration postinfection as a measure of fish diarrhea. The bar diagrams show the mucin level in excreted water after 24 h. (D) Bacterial numbers in the water postinfection were quantified to determine the levels of excreted bacteria. ***, P < 0.001. Error bars represent ± standard error of the means in panel B and standard deviations in panels C and D.