FIG 5.

³¹P NMR spectra of small molecules extracted from 2-day (A), 6-day (B), 51-day (C), and 91-day (D) spores. Small molecules were extracted from spores of various ages, and extracts were processed and examined by ³¹P NMR, as described in Materials and Methods. Labeled peaks are from 3PGA, AMP, and P_i, and all spectra are shown at the same scale. There appear to be slightly broader peaks for P_i and 3PGA in spectra from 6- and 51-day spores, perhaps because of less efficient Mn²⁺ removal from these samples, and this could contribute to lower peak heights in these samples. a.u., arbitrary units.