TABLE 2.
Relative levels of mRNAs in spores aged for 2, 47, or 98 daysa
| Gene | No. of individual mRNA reads/total no. of reads for 23S + 16S rRNAs × 106 by spore age |
||
|---|---|---|---|
| 2 days | 47 days | 98 days | |
| sspA | 68 | 6 | 4 |
| sspE | 249 | 83 | 62 |
| sspF | 872 | 15 | 4 |
| sspJ | 131 | 9 | 3 |
| sspK | 191 | 2 | 0 |
| sspM | 61 | 1 | 0 |
| sspN | 148 | 15 | 2 |
| tlp | 150 | 8 | 3 |
| sspO | 34 | 3 | 0 |
| sspP | 389 | 1 | 1 |
| yfhD | 1,289 | 29 | 7 |
| yhcN | 320 | 1 | 1 |
| yhcQ | 192 | 7 | 3 |
| yhcV | 1,546 | 84 | 16 |
| yhdB | 1,458 | 4 | 1 |
| yizC | 719 | 27 | 4 |
| ykzE | 353 | 20 | 4 |
| ykzP | 697 | 38 | 1 |
| ymfJ | 421 | 15 | 1 |
| yozQ | 13 | 2 | 1 |
| ypzF | 1,791 | 25 | 10 |
| ypzG | 3,646 | 84 | 16 |
| yqfX | 1,080 | 18 | 0 |
| yrzQ | 293 | 2 | 0 |
| yrzR | 303 | 2 | 1 |
| ytzC | 504 | 61 | 12 |
| ytzL | 950 | 7 | 1 |
| yuzA | 72 | 2 | 0 |
| yxeD | 27 | 3 | 1 |
| sspB | 31 | 1 | 3 |
| sspH | 20 | 0 | 0 |
| yhcM | 576 | 1 | 1 |
| yraE | 52 | 0 | 0 |
| yrrD | 264 | 15 | 0 |
| yusG | 11 | 0 | 0 |
| yusN | 4 | 0 | 0 |
| pdhA | 12 | 0 | 0 |
| sscA | 1 | 0 | 0 |
| sspI | 19 | 0 | 0 |
| yhcO | 112 | 2 | 0 |
| ykzD | 9 | 0 | 0 |
| yoyE | 79 | 2 | 0 |
| ypzI | 3 | 0 | 0 |
| yraD | 12 | 1 | 0 |
| yrzB | 1 | 0 | 0 |
| yutC | 13 | 0 | 0 |
| Total no. of mRNA reads/no. of 23S + 16S rRNA reads × 106 (%)b | 19,041 (100) | 566 (3) | 163 (0.9) |
| No. of 23S + 16S rRNA reads | 94,820,503 | 77,821,429 | 83,264,436 |
a
Spores of various ages were prepared and purified, RNA was extracted, RNA-seq was carried out, and the data were analyzed, as described in Materials and Methods.
b
Values in parentheses are the percentages of total number of mRNA reads/number of 23S + 16S rRNA reads relative to the value in 2-day spores.