FIG 3.

Role of TNF secretion in LegC4-mediated attenuation of L. pneumophila replication. (A and B) ELISA quantification of TNF (A) or IL-6 (B) secretion from wild-type BMDMs infected with L. pneumophila ΔflaA ΔlegC4(pEV) and ΔflaA ΔlegC4(plegC4) at the indicated time points. (C) Enumeration of L. pneumophila strains from BMDMs assayed for panels A and B. (D) Fold replication over 72 h of the indicated L. pneumophila strains within wild-type or Myd88^−/− BMDMs. Expression of legC4 was induced with 1 mM IPTG. Data shown are mean ± SD for samples in triplicate. Asterisks denote statistical significance (*, P < 0.05; **, P < 0.01) by Student’s t test. Data are representative of at least two independent experiments.